rabbit polyclonal antibody against brd3 Search Results


a302 368a rrid ab 1907251  (Bethyl)
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Bethyl a302 368a rrid ab 1907251

A302 368a Rrid Ab 1907251, supplied by Bethyl, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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brd3  (Proteintech)
93
Proteintech brd3
Effects of JQ1 and BMN673 on the HR repair pathway. (A) Representative images of Rad51 immunofluorescence staining in SCLC cells treated with drugs as indicated for 24 h. Scale bar, 20 μm. Quantification of Rad51 fluorescence intensities from three independent experiments was shown as mean ± S.D. **P < 0.01; ***P < 0.001. (B) Western blot analysis of Rad51 expression in SCLC cells treated with drugs as indicated for 24 h. (C) Western blot analysis of indicated proteins in DMS273 cell upon knockdown of BRD2 or <t>BRD3</t> or BRD4 . (D) ChIP-PCR analysis displays the decreased association of BRD4 in the promoter of RAD51 in DMS273 and H526 cells treatment with JQ1 for 24 h. *P < 0.05; **P < 0.01; ns, no significance. (E) Western blot analysis of phosphorylated-DNA-PKcs and phosphorylated-RPA32 in MYC paralog-dependent and independent-SCLC cells treated with indicated drugs for 24 h. β-actin was used as a loading control.
Brd3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti brd3  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology mouse anti brd3
Effects of JQ1 and BMN673 on the HR repair pathway. (A) Representative images of Rad51 immunofluorescence staining in SCLC cells treated with drugs as indicated for 24 h. Scale bar, 20 μm. Quantification of Rad51 fluorescence intensities from three independent experiments was shown as mean ± S.D. **P < 0.01; ***P < 0.001. (B) Western blot analysis of Rad51 expression in SCLC cells treated with drugs as indicated for 24 h. (C) Western blot analysis of indicated proteins in DMS273 cell upon knockdown of BRD2 or <t>BRD3</t> or BRD4 . (D) ChIP-PCR analysis displays the decreased association of BRD4 in the promoter of RAD51 in DMS273 and H526 cells treatment with JQ1 for 24 h. *P < 0.05; **P < 0.01; ns, no significance. (E) Western blot analysis of phosphorylated-DNA-PKcs and phosphorylated-RPA32 in MYC paralog-dependent and independent-SCLC cells treated with indicated drugs for 24 h. β-actin was used as a loading control.
Mouse Anti Brd3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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brd4 a301 985a100  (Bethyl)
96
Bethyl brd4 a301 985a100
Effects of JQ1 and BMN673 on the HR repair pathway. (A) Representative images of Rad51 immunofluorescence staining in SCLC cells treated with drugs as indicated for 24 h. Scale bar, 20 μm. Quantification of Rad51 fluorescence intensities from three independent experiments was shown as mean ± S.D. **P < 0.01; ***P < 0.001. (B) Western blot analysis of Rad51 expression in SCLC cells treated with drugs as indicated for 24 h. (C) Western blot analysis of indicated proteins in DMS273 cell upon knockdown of BRD2 or <t>BRD3</t> or BRD4 . (D) ChIP-PCR analysis displays the decreased association of BRD4 in the promoter of RAD51 in DMS273 and H526 cells treatment with JQ1 for 24 h. *P < 0.05; **P < 0.01; ns, no significance. (E) Western blot analysis of phosphorylated-DNA-PKcs and phosphorylated-RPA32 in MYC paralog-dependent and independent-SCLC cells treated with indicated drugs for 24 h. β-actin was used as a loading control.
Brd4 A301 985a100, supplied by Bethyl, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti-brd3  (Active Motif)
90
Active Motif rabbit anti-brd3
Effects of JQ1 and BMN673 on the HR repair pathway. (A) Representative images of Rad51 immunofluorescence staining in SCLC cells treated with drugs as indicated for 24 h. Scale bar, 20 μm. Quantification of Rad51 fluorescence intensities from three independent experiments was shown as mean ± S.D. **P < 0.01; ***P < 0.001. (B) Western blot analysis of Rad51 expression in SCLC cells treated with drugs as indicated for 24 h. (C) Western blot analysis of indicated proteins in DMS273 cell upon knockdown of BRD2 or <t>BRD3</t> or BRD4 . (D) ChIP-PCR analysis displays the decreased association of BRD4 in the promoter of RAD51 in DMS273 and H526 cells treatment with JQ1 for 24 h. *P < 0.05; **P < 0.01; ns, no significance. (E) Western blot analysis of phosphorylated-DNA-PKcs and phosphorylated-RPA32 in MYC paralog-dependent and independent-SCLC cells treated with indicated drugs for 24 h. β-actin was used as a loading control.
Rabbit Anti Brd3, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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brd2 rabbit bethyl bl167 2a2  (Bethyl)
91
Bethyl brd2 rabbit bethyl bl167 2a2
Effects of JQ1 and BMN673 on the HR repair pathway. (A) Representative images of Rad51 immunofluorescence staining in SCLC cells treated with drugs as indicated for 24 h. Scale bar, 20 μm. Quantification of Rad51 fluorescence intensities from three independent experiments was shown as mean ± S.D. **P < 0.01; ***P < 0.001. (B) Western blot analysis of Rad51 expression in SCLC cells treated with drugs as indicated for 24 h. (C) Western blot analysis of indicated proteins in DMS273 cell upon knockdown of BRD2 or <t>BRD3</t> or BRD4 . (D) ChIP-PCR analysis displays the decreased association of BRD4 in the promoter of RAD51 in DMS273 and H526 cells treatment with JQ1 for 24 h. *P < 0.05; **P < 0.01; ns, no significance. (E) Western blot analysis of phosphorylated-DNA-PKcs and phosphorylated-RPA32 in MYC paralog-dependent and independent-SCLC cells treated with indicated drugs for 24 h. β-actin was used as a loading control.
Brd2 Rabbit Bethyl Bl167 2a2, supplied by Bethyl, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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western blot santa cruz sc 8349 rrid ab 2253533 brd3  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc western blot santa cruz sc 8349 rrid ab 2253533 brd3
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Western Blot Santa Cruz Sc 8349 Rrid Ab 2253533 Brd3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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brd3  (Bethyl)
95
Bethyl brd3
BRD4 depletion inhibits DUX4 expression. a – c Expression of BET genes ( a ), BET proteins ( b ), and DUX4 and DUX4 targets ( c ) after BRD2 , <t>BRD3</t> , or BRD4 siRNA knockdown in 54-2 FSHD1 myoblasts. Error bars indicate the standard deviation from the mean of three biological replicates. GAPDH serves as a loading control. p values were calculated using a one-way analysis of variance with Dunnett’s post test. * p < 0.05
Brd3, supplied by Bethyl, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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brd3 sc 81802 santa cruz wb  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology brd3 sc 81802 santa cruz wb
BRD4 depletion inhibits DUX4 expression. a – c Expression of BET genes ( a ), BET proteins ( b ), and DUX4 and DUX4 targets ( c ) after BRD2 , <t>BRD3</t> , or BRD4 siRNA knockdown in 54-2 FSHD1 myoblasts. Error bars indicate the standard deviation from the mean of three biological replicates. GAPDH serves as a loading control. p values were calculated using a one-way analysis of variance with Dunnett’s post test. * p < 0.05
Brd3 Sc 81802 Santa Cruz Wb, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-brd3  (Thermo Fisher)
90
Thermo Fisher anti-brd3
BRD4 depletion inhibits DUX4 expression. a – c Expression of BET genes ( a ), BET proteins ( b ), and DUX4 and DUX4 targets ( c ) after BRD2 , <t>BRD3</t> , or BRD4 siRNA knockdown in 54-2 FSHD1 myoblasts. Error bars indicate the standard deviation from the mean of three biological replicates. GAPDH serves as a loading control. p values were calculated using a one-way analysis of variance with Dunnett’s post test. * p < 0.05
Anti Brd3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse monoclonal antibody  (Thermo Fisher)
90
Thermo Fisher mouse monoclonal antibody
BRD4 depletion inhibits DUX4 expression. a – c Expression of BET genes ( a ), BET proteins ( b ), and DUX4 and DUX4 targets ( c ) after BRD2 , <t>BRD3</t> , or BRD4 siRNA knockdown in 54-2 FSHD1 myoblasts. Error bars indicate the standard deviation from the mean of three biological replicates. GAPDH serves as a loading control. p values were calculated using a one-way analysis of variance with Dunnett’s post test. * p < 0.05
Mouse Monoclonal Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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brd3  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc brd3
BRD4 depletion inhibits DUX4 expression. a – c Expression of BET genes ( a ), BET proteins ( b ), and DUX4 and DUX4 targets ( c ) after BRD2 , <t>BRD3</t> , or BRD4 siRNA knockdown in 54-2 FSHD1 myoblasts. Error bars indicate the standard deviation from the mean of three biological replicates. GAPDH serves as a loading control. p values were calculated using a one-way analysis of variance with Dunnett’s post test. * p < 0.05
Brd3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Journal: Molecular Cell

Article Title: Bromodomain Protein BRD4 Is a Transcriptional Repressor of Autophagy and Lysosomal Function

doi: 10.1016/j.molcel.2017.04.027

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-BRD3 , Bethyl Laboratories , Cat#: A302-368A; RRID: AB_1907251.

Techniques: Control, Membrane, Virus, Recombinant, Sample Prep, Flow Cytometry, Western Blot, Microscopy, Plasmid Preparation, Phospho-proteomics, Variant Assay, shRNA, Software, CRISPR

Effects of JQ1 and BMN673 on the HR repair pathway. (A) Representative images of Rad51 immunofluorescence staining in SCLC cells treated with drugs as indicated for 24 h. Scale bar, 20 μm. Quantification of Rad51 fluorescence intensities from three independent experiments was shown as mean ± S.D. **P < 0.01; ***P < 0.001. (B) Western blot analysis of Rad51 expression in SCLC cells treated with drugs as indicated for 24 h. (C) Western blot analysis of indicated proteins in DMS273 cell upon knockdown of BRD2 or BRD3 or BRD4 . (D) ChIP-PCR analysis displays the decreased association of BRD4 in the promoter of RAD51 in DMS273 and H526 cells treatment with JQ1 for 24 h. *P < 0.05; **P < 0.01; ns, no significance. (E) Western blot analysis of phosphorylated-DNA-PKcs and phosphorylated-RPA32 in MYC paralog-dependent and independent-SCLC cells treated with indicated drugs for 24 h. β-actin was used as a loading control.

Journal: Frontiers in Oncology

Article Title: The MYC Paralog-PARP1 Axis as a Potential Therapeutic Target in MYC Paralog-Activated Small Cell Lung Cancer

doi: 10.3389/fonc.2020.565820

Figure Lengend Snippet: Effects of JQ1 and BMN673 on the HR repair pathway. (A) Representative images of Rad51 immunofluorescence staining in SCLC cells treated with drugs as indicated for 24 h. Scale bar, 20 μm. Quantification of Rad51 fluorescence intensities from three independent experiments was shown as mean ± S.D. **P < 0.01; ***P < 0.001. (B) Western blot analysis of Rad51 expression in SCLC cells treated with drugs as indicated for 24 h. (C) Western blot analysis of indicated proteins in DMS273 cell upon knockdown of BRD2 or BRD3 or BRD4 . (D) ChIP-PCR analysis displays the decreased association of BRD4 in the promoter of RAD51 in DMS273 and H526 cells treatment with JQ1 for 24 h. *P < 0.05; **P < 0.01; ns, no significance. (E) Western blot analysis of phosphorylated-DNA-PKcs and phosphorylated-RPA32 in MYC paralog-dependent and independent-SCLC cells treated with indicated drugs for 24 h. β-actin was used as a loading control.

Article Snippet: Antibodies against the following proteins were used for our studies: c-MYC (1:1,000, abcam ab32072), PARP1 (1:1,000, Affinity DF7198), GAPDH (1:10,000, Affinity AF7021), Rad51 (1:10,000, Abcam ab133534), PARP (1:1,000, CST 9542), γH2AX (1:1,000, CST 2577), p-CHK1 (1:1,000, Ser317, CST 12302), BRD2 (1:1,000, Proteintech 22236-1-AP), BRD3 (1:1,000, Proteintech 11859-1-AP), BRD4 (1:500, Bethyl A301-985A50), p-DNA-PKcs (1:1,000, Abcam ab124918), p-RPA32 (1:5,000, Ser4/Ser8, Novus), MYCN (1:1,000, CST 9405), MYCL (1:1,000 R&D, AF4050) and β-actin (1:10,000, Transgen HC201-02).

Techniques: Immunofluorescence, Staining, Fluorescence, Western Blot, Expressing, Knockdown, Control

KEY RESOURCES TABLE

Journal: Cancer cell

Article Title: BRD4 inhibition is synthetic lethal with PARP inhibitors through the induction of homologous recombination deficiency

doi: 10.1016/j.ccell.2018.01.019

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies BRCA1 (D-9) antibody Santa Cruz sc-6954, RRID:AB_626761 ERK 2 (C-14) antibody Santa Cruz sc-154, RRID:AB_2141292 RAD51 (H-92) antibody for Western blot Santa Cruz sc-8349, RRID:AB_2253533 BRD3 (2088C3a) antibody Santa Cruz sc-81202, RRID:AB_1119692 CtIP (D76F7) Rabbit mAb antibody Cell Signaling Technology #9201S, RRID:AB_10828593 MRE11 (31H4) Rabbit mAb antibody Cell Signaling Technology #4847S, RRID:AB_10693469 53BP1 Antibody Cell Signaling Technology #4937S, RRID:AB_10694558 BRD4 (E2A7X) Rabbit Antibody Cell Signaling Technology #13440S, RRID:AB_2687578 BRD2 (D89B4) Rabbit mAb antibody Cell Signaling Technology #5848S, RRID:AB_10835146 RAD51 (Ab-1) Rabbit pAb antibody for immunofluorescence and IHC Millipore Corp PC130, RRID:AB_2238184 Histone H2A.X, phospho (Ser139) Monoclonal antibody, Unconjugated, Clone jbw301 Millipore Corp 05-636, RRID:AB_309864 RPA32/RPA2 antibody [9H8] Abcam ab2175, RRID:AB_302873 RAD50 antibody [EPR3466(2)] Abcam ab124682, RRID:AB_11000808 NBS1 antibody [Y112] - ChIP Grade Abcam ab32074, RRID:AB_777007 Phospho RPA32 (S4/S8) Antibody Bethyl Laboratories A300-245A, RRID:AB_210547 Phospho RPA32 (S33) Antibody Bethyl Laboratories A300-246A, RRID:AB_2180847 RPA70 antibody [EPR3472] Abcam ab79398, RRID:AB_1603759 Histone H3 antibody - ChIP Grade Abcam ab1791, RRID:AB_302613 H3K27ac-human antibody Abcam ab4729, RRID:AB_2118291 PARP-1 (H-250) antibody Santa Cruz sc-7150, RRID:AB_2160738 Pol II (CTD4H8) antibody Santa Cruz sc-47701, RRID:AB_677353 Histone H3 (mono methyl K4) antibody - ChIP Grade Abcam ab8895, RRID:AB_306847 normal Rabbit IgG Cell Signaling Technology #2729.

Techniques: Western Blot, Immunofluorescence, Recombinant, Staining, Lysis, SYBR Green Assay, Transfection, Plasmid Preparation, In Vitro, In Vivo, Mutagenesis, Chromatin Immunoprecipitation, Fractionation, Cell Culture, Expressing, Inhibition, Software

BRD4 depletion inhibits DUX4 expression. a – c Expression of BET genes ( a ), BET proteins ( b ), and DUX4 and DUX4 targets ( c ) after BRD2 , BRD3 , or BRD4 siRNA knockdown in 54-2 FSHD1 myoblasts. Error bars indicate the standard deviation from the mean of three biological replicates. GAPDH serves as a loading control. p values were calculated using a one-way analysis of variance with Dunnett’s post test. * p < 0.05

Journal: Skeletal Muscle

Article Title: BET bromodomain inhibitors and agonists of the beta-2 adrenergic receptor identified in screens for compounds that inhibit DUX4 expression in FSHD muscle cells

doi: 10.1186/s13395-017-0134-x

Figure Lengend Snippet: BRD4 depletion inhibits DUX4 expression. a – c Expression of BET genes ( a ), BET proteins ( b ), and DUX4 and DUX4 targets ( c ) after BRD2 , BRD3 , or BRD4 siRNA knockdown in 54-2 FSHD1 myoblasts. Error bars indicate the standard deviation from the mean of three biological replicates. GAPDH serves as a loading control. p values were calculated using a one-way analysis of variance with Dunnett’s post test. * p < 0.05

Article Snippet: The following antibodies were used: α-Tubulin (DM1A), Sigma-Aldrich T9026; β2-AR (R11E1), Santa Cruz Biotechnology (Dallas, TX, USA) sc-81577, lot#G1117; BRD2, Bethyl Laboratories (Montgomery, TX, USA) A302-582A; BRD3, Bethyl A302-368A; BRD4, Bethyl A301-985A50; GAPDH, Thermo Fisher Scientific TAB1001; Histone H3, Abcam (Cambridge, UK) ab1791; and MBD3L2, Abcam ab107999, lot#GR126890-1; rabbit monoclonal antibody against DUX4 (E14-3) was produced in collaboration with Epitomics (Burlingame, CA, USA) and is described elsewhere [ ].

Techniques: Expressing, Knockdown, Standard Deviation, Control